Colorimetric Method for Detecting Amplified Nucleic Acids

Colorimetric method for detecting amplified nucleic acids.

Methods for detecting and measuring the success of nucleic acid sequence amplifications can be developed by detecting the by-products of amplification procedures. One method includes the detection of inorganic phosphate (Pi) during or on completion of the PCR. The method requires modification of assay conditions to prevent thermal- and template-independent enzymatic activity from nonspecificall...

Modular Assembly of a Pd Catalyst within a DNA Scaffold for the Amplified Colorimetric and Fluorimetric Detection of Nucleic Acids**

Catalytic signal amplification is a powerful tool for the detection of chemical and biological analytes. In chemistry it has been employed in sensing various toxic metal ions (e.g. Pd, Pb, Cu, and Hg) as well as small molecules such as carbon monoxide and thiols. In a biological context, catalytic reactions have enabled the highly sensitive detection of scarce analytes. They have been widely ut...

Nonradioactive, colorimetric microplate hybridization assay for detecting amplified human immunodeficiency virus DNA.

A nonradioactive, colorimetric microplate hybridization procedure was used to assay human immunodeficiency virus (HIV) DNA, amplified by the polymerase chain reaction (PCR). Under the PCR conditions used, four proviral copies per 150,000 cells were detected by amplifying a series of DNA mixtures that contained various copy numbers of HIV. Assays of PCR-amplified DNA from peripheral blood mononu...

Colorimetric Ultramicro Method for the Determination of Free Fatty Acids.

Enzymatically amplified surface plasmon resonance imaging method using RNase H and RNA microarrays for the ultrasensitive detection of nucleic acids.

A novel surface enzymatic amplification method that utilizes RNA microarrays in conjunction with the enzyme RNase H is developed for the ultrasensitve detection and analysis of target DNA molecules. The enzyme RNase H is shown to selectively and repeatedly destroy RNA from RNA-DNA heteroduplexes on gold surfaces; when used in conjunction with the label-free technique of surface plasmon resonanc...